Crosslink Cells [ download protocol ]

We first fix endogenous lncRNA-chromatin interactions by crosslinking cells. We generally use a combination of formaldehyde and the protein-protein crosslinker disuccinimidyl glutarate (DSG), although RNA purification with RAP also works with cells fixed with other crosslinking reagents.

DSG Crosslinking

Formaldehyde Crosslinking

Harvest Cells