Design Capture Probes [ download protocol ] [ download Probe Design Tool]

A. Probe Design

B. Enrichment PCR

Enrich for particular probe-sets using primers specific to the incorporated sequences

Set up PCR reaction on ice:

IVT Mix

1 Reaction

Agilent Oligo Pool (10nM)

0.2μl

H2O

3.8μl

Enrich Primer Mix (5uM each)

1μl

Phusion HF 2x Master Mix

5μl

Total

10μl

PCR:  98C 30 seconds, 25x [98C 10s, 62C 20s, 72C 20s], 72C 60 s, 4C hold.

C. SILANE Clean-up