Wash and Elute [ download protocol ]

The strong interactions between the probes and target RNA enables washing in high stringency conditions (3M guanidine thiocyanate at 45°C), thus reducing the potential for false positives due to non-specific interactions. Finally, we elute bound RNA-chromatin complexes and sequence the purified genomic DNA.

A. Wash beads

B. Elution and Reverse Crosslinking

C. Aliquot Samples

D. SPRI Cleanup